Munc13 Clusters Capture Vesicles to Lipid Bilayer Membrane

Formation and properties of cell-size lipid bilayer vesicles.

Hydration of single or mixed phospholipids or lipid protein mixtures at low ionic strength results in the formation of a population of large, solvent free, single bilayer vesicles with included volumes of up to 300 microliters/mumol lipid. Their size ranges from 0.1 to 300 microns and they can be sorted out according to size by centrifugation. When formed in distilled water their internal solut...

Imaging Lipid Microdomains in a Microfluidic Bilayer Lipid Membrane Chip

A microfluidic bilayer lipid membrane (BLM) system supporting correlated opto-electrical membrane characterization has been developed and applied to the imaging of phase-separated lipid microdomains by confocal microscopy. The device consists of a microfluidic network supporting rapid perfusion fabricated in a thermoplastic substrate, bonded to a thin PVDC partition and supported by a thin cove...

Rheological Behavior of a Dispersion of Small Lipid Bilayer Vesicles

Rheological behavior of a dispersion of small nearly-unilamellar phospholipid bilayer vesicles has been investigated. We conducted steady-state shear experiments and linear viscoelastic experiments. In the dilute and semidilute regime the rheological behavior is similar to that of a hard-sphere dispersion as reported in the literature for viscoelasticmeasurements, butnowalso observed in steady ...

3. Bilayer Adaptability and Elasticity of Mixed Lipid Vesicles, Transfersome

Vesicles surfing on a lipid bilayer: self-induced haptotactic motion.

Haptotaxis is a mechanism proposed at the end of the 1960s to explain cell motility. It describes cell movement induced by an adhesion gradient. In this work, we present evidence for self-induced haptotaxis using negatively charged giant vesicles interacting with positively charged supported lipid bilayers, which has not been previously described. Depending on the charge of the vesicle, we obse...